FastQCFastQC Report
Sun 14 Apr 2019
SRR6576225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445985
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13340.29911319887440163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.20337006850006167No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8380.18789869614448917No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7490.16794286803367825No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7450.1670459768826306No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6860.15381683240467728No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6730.15090193616377232No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6430.14417525253091473No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6420.1439510297431528No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6400.14350258416762895No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6220.1394665739879144No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6080.1363274549592475No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5850.13117033084072333No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT5500.12332253326905614No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5080.11390517618305548No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC5030.11278406224424589No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC5020.11255983945648396No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4990.11188717109319819No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4840.1085238292767694No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG4780.10717849255019787No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4720.10583315582362635No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4690.1051604874603406No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4670.10471204188481677No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4520.10134870006838796No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4470.10022758612957836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG451.6126867E-552.2203565
GTATCAA14350.045.6627351
ATCAACG15950.039.189823
TCAACGC16300.038.6366544
GTATAGG2700.038.411211
CAACGCA16350.037.9435965
ACACCGC500.001701209537.5986566
AACGCAG16500.037.5986566
CTACAGT500.001701209537.5986564
TTAAGAC651.4124005E-436.1525543
TGACCGT851.7528138E-533.1752855
ACCGTGC851.7528138E-533.1752857
ATGGGAT4050.032.4926645
GTACATG23100.031.4273511
CTTATTG753.273039E-431.3322143
GGTATAG902.5910267E-531.3322127
TACCCAT1201.6489685E-731.332215
AGACGAC300.004161215431.3322188-89
GTATACC600.00415900531.332214
GACGCCC600.00415900531.332218
Produced by FastQC (version 0.11.2)