FastQCFastQC Report
Sun 14 Apr 2019
SRR6576228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395289
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7180.18163925634156275No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3970.10043284786573875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA301.3700718E-462.66655
CCGCTAA405.6722475E-446.9998744
GTATCAA8500.043.1347541
TACATGG24250.041.8691022
GTACATG24700.041.486921
ACATGGG24750.039.878683
CATGGGT7000.035.5856174
CATGGGA11300.034.522034
AACGCAG11050.032.3257066
TCAACGC10950.032.1916964
ATCAACG11000.032.0453723
ATGGGAT3550.031.774565
GGTATAG600.004155190731.3372121
CAACGCA11300.031.1946055
ATGGGTA3800.029.6841325
GGTACCT3600.028.7221458
ATGGGTG3450.027.2463045
ATGGGAG6400.027.1718035
CATGGGG9400.026.999934
TATCAAC13700.026.7624382
Produced by FastQC (version 0.11.2)