FastQCFastQC Report
Sun 14 Apr 2019
SRR6576228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395289
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7030.17784456435671117No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4400.11131096488897997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10050.045.528441
ACACTCG450.001013342241.7761466
CCGATCA250.001702032337.59853432-33
TACATGG23500.036.9985542
GTACATG24200.036.255561
ATCAACG12200.035.826473
ACATGGG24000.035.05283
CAACGCA12700.034.7860455
TCAACGC12600.034.689124
AACGCAG13150.033.5956466
CATGGGT6250.032.334744
TATCAAC14350.031.7687952
ACGCAGA14550.030.3630777
GTGTAAT1401.8188075E-830.3243521
CGCAGAG14750.029.6327428
CATGGGG9600.028.884294
GCAGAGT16450.027.1417989
GTAGGAC1258.008941E-626.3189723
GCTATCC1501.1597003E-625.065693
GGTATCA5200.024.4927461
Produced by FastQC (version 0.11.2)