FastQCFastQC Report
Sun 14 Apr 2019
SRR6576229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356883
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6770.1896980242824679No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4090.11460338542323395No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3650.10227441486425524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT405.667509E-447.0065841
GTATCAA7700.045.1751631
TACATGG21600.041.1307682
GTACATG21700.040.7245981
ACATGGG22150.039.668343
GTACTAG1003.311834E-837.605271
CATGGGT5900.037.4301874
ATCAACG9450.036.793923
AACGCAG9800.036.4387636
TCAACGC9750.035.6617974
CAACGCA10150.034.719335
ATGGGTA3950.034.496665
CATGGGA10750.033.2185944
GGGTACC3100.031.8297887
TGCCCGC1201.6448575E-731.3377271
ACGCAGA12000.029.7583267
TATCAAC12300.029.0447222
CGCAGAG12300.029.0325158
GGTACCT3400.029.0212788
GTACGGT650.00614434928.9271321
Produced by FastQC (version 0.11.2)