FastQCFastQC Report
Sun 14 Apr 2019
SRR6576230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444095
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11080.24949616636080119No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6530.14704061068014726No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5890.1326292797712201No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5770.1299271552257963No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5070.11416476204415722No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4840.10898568999876151No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4820.10853533590785755No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4730.10650874249878968No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4480.10087931636249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG301.370434E-462.6648677
GTATCAA11500.041.3515741
GTACATG28000.038.675711
TACATGG28350.038.4609762
ACATGGG28400.037.5658233
CATGGGG11650.035.5011254
ATGGGGA6450.032.0610925
AACGCAG14300.031.8802016
ATCAACG14350.031.7691233
ATGGGAG5850.030.5290375
TCAACGC15050.030.2914894
CAACGCA15250.029.8942225
ATACTAA953.7478843E-529.6833556
ACGCAGA16200.027.8510517
CGCAGAG16400.027.7979838
CATGGGA14850.027.5345634
TATCAAC17800.025.875662
CATGGGT6700.025.2530064
ATGGGTA4600.024.5210345
GCAGAGT19550.023.7998269
Produced by FastQC (version 0.11.2)