FastQCFastQC Report
Sun 14 Apr 2019
SRR6576231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399250
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10630.2662492172824045No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5730.14351909830933No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5440.13625547902316842No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5070.12698810269254854No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4830.12097683155917345No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4690.11747025673137133No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4610.11546649968691297No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4580.11471509079524107No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4230.10594865372573577No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4150.1039448966812774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.037.8251951
GTATATC651.413233E-436.1477783
GTACATG25750.035.2964171
ATGGGTA4550.035.1149835
TACATGG25650.034.9921762
GGTACCT4750.034.647468
ACATGGG25800.034.606593
GGGTACC4800.032.307077
AACGCAG14450.031.5448786
GTATAAG902.5551934E-531.3949131
CATGGGT6800.031.0977214
TATACAG2000.030.5448725
TCAACGC14950.030.1755374
CAACGCA15250.029.8900625
ATCAACG15100.029.8757783
CCTATAC953.750505E-529.6792283
TGGGTAC5250.029.53796
ATTAGGC804.7800568E-429.370073
GTCTATA650.006090706728.979921
TATAGGA1303.3272954E-728.9182222