FastQCFastQC Report
Sun 14 Apr 2019
SRR6576234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329846
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5350.16219690400975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.13703364600449905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3680.11156721621605234No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3450.10459426520254907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.047.436041
CCCTATA901.2732926E-841.908261
GTAGGAC450.001011745341.7874873
GTACATG19300.038.5968551
ACATGGG19800.037.5137673
TACATGG19850.037.4192732
ATCAACG10250.036.691453
CATGGGG8450.035.6059044
TCAACGC10750.034.9848674
AACGCAG10750.034.5475586
CAACGCA11500.032.7032475
CATGGGA8250.031.9104424
TCTAATA753.266063E-431.3406122
TATCAAC12350.031.2137282
ATGGGGA4600.030.6592965
CATGGGT4950.029.4411834
GGTATCA4050.029.1029571
GACTATT650.00615458328.916647
ACGCAGA13000.028.5551827
CGCAGAG13300.027.5577758
Produced by FastQC (version 0.11.2)