FastQCFastQC Report
Sun 14 Apr 2019
SRR6576235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306433
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5290.1726315377260282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.11128044303322424No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3340.10899609376274749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA250.005234975456.392955
GTATCAA7550.044.222021
CGATAAT250.00170381837.58916566-67
TACATGG17600.033.1365362
GTACATG17850.032.930551
ATCAACG9850.032.447943
CATGGGG8100.031.91484
AACGCAG10350.031.7834686
ACATGGG18200.031.764953
TCAACGC10150.031.488894
CAACGCA10350.030.8753685
CTAGTAC650.006146070528.9241833
ATGGGAG3100.028.802855
CCATAGA1154.1660314E-628.6238061
ACGCAGA11550.028.0744117
CGCAGAG11750.027.596558
TATCAAC12350.027.038932
TGCCCGC1257.966079E-626.3339021
GTATTAT909.4724406E-426.1249051
CCTAGTA950.001297163924.753952
Produced by FastQC (version 0.11.2)