FastQCFastQC Report
Sun 14 Apr 2019
SRR6576235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306433
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.1677365035750066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.13445027134805979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3940.12857623036683385No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3650.11911249767485878No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3110.10149037473118104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA250.005217044656.4418594
GTATCAA8350.043.9870641
GTACATG18500.037.9255071
TATAGGT500.001702067437.5910577
TACATGG19000.036.366382
AACGCAG10200.035.471956
ACATGGG19250.035.3999523
TCAACGC10750.034.099784
ATCAACG10900.033.1993563
ATAGACT851.7514969E-533.1739964
CAACGCA11050.032.7486845
CATGGGT5150.031.9393624
TTAGTAC600.00415778131.3309963
ATGGGTG2100.031.3309925
ACGCAGA12300.029.0337437
CATGGGA9150.028.7628784
CGCAGAG12700.028.1422568
TATCAAC13100.027.9872132
ATAATGG2156.548362E-1126.23063
CATGGGG8200.025.7907564