FastQCFastQC Report
Sun 14 Apr 2019
SRR6576236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489777
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7190.1468015035414076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.12924249199125318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.048.518611
GGTATCA4450.039.2330361
TTACGGG500.001701464437.5982444
ACATGGG21100.035.415413
ATAAGAC1204.1982275E-935.2483563
TACATGG21750.035.2357332
CAACGCA12550.035.201545
TCAACGC12500.034.5903854
GTACATG22050.034.4530371
ATCAACG12850.032.916753
AACGCAG13450.032.846056
CCGATTA300.00416163631.33187118-19
CTTACGG600.00415962331.3318713
CATGGGT5300.031.0362854
CATGGGG12350.030.4439244
ACGCAGA15450.028.5941337
TATCAAC15200.028.457662
CCCTATA1005.304288E-528.2102012
CGCAGAG16450.026.8558888
CATGGGA8700.023.22884
Produced by FastQC (version 0.11.2)