FastQCFastQC Report
Sun 14 Apr 2019
SRR6576239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495626
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8540.172307344651007No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.11197959751909706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.040.823241
TACATGG35000.035.995142
ACATGGG34650.035.9444733
GTACATG35100.035.6247331
AACGCAG18750.032.5860376
CAACGCA19150.031.9053885
ATCAACG19200.031.8223043
TCAACGC19250.031.7396474
ATGGGAG9150.031.3327275
CATGGGT7100.031.1120744
GTATAGG1401.8830178E-830.2197991
GTTATAC804.7775463E-429.3744343
CATGGGA20700.029.062244
CATGGGG11400.027.6222724
TATCAAC22550.027.5172182
GGTACCT3800.027.218
ACGCAGA22600.027.0348787
CGCAGAG23150.026.3925788
GGGTACC4200.025.7375987
TGGGTAC4500.025.0661836
Produced by FastQC (version 0.11.2)