FastQCFastQC Report
Sun 14 Apr 2019
SRR6576240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446760
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8470.18958725042528426No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5270.11796042617960427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.11482675261885578No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4780.10699256871698451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG201.8394652E-593.971134
GTATCAA14400.039.1677861
ACATGGG29600.037.778933
GTACATG30500.037.4469761
TACATGG30300.037.3839072
GTATATA2050.034.3912281
CATGGGT5750.032.6856084
ATGGGTA3650.032.1818925
CATGGGG9700.031.9695554
AACGCAG17800.031.675666
TTAGGGT1201.6528975E-731.323714
TATACGG753.2782627E-431.3237085
ATCAACG18100.030.891063
TCAACGC18350.030.47024
CAACGCA18500.030.2231465
TCTATAC1102.9701969E-629.8999043
CATGGGA17900.028.8738124
ACTATCT1154.198875E-628.5999098
ACGCAGA20800.026.8811637
TATCAAC20850.026.600282
Produced by FastQC (version 0.11.2)