FastQCFastQC Report
Sun 14 Apr 2019
SRR6576241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481753
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10410.21608583651788363No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6910.1434344985916019No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5760.11956334470153793No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5730.11894061894788409No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5620.11665729118448666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.11582699017961486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10650.054.449431
TAACGCA405.674344E-446.9984444
TCAACGC14050.038.8029864
ATCAACG14150.038.528763
CAACGCA14250.038.258385
GTACATG25300.037.268961
AACGCAG14950.036.781396
TACATGG26050.035.902842
ACATGGG25950.035.678973
TATCAAC16450.033.713172
ACGCAGA17350.031.6934767
CGCAGAG17750.031.2440368
CATGGGT6550.030.8539414
CATGGGA10150.030.5605644
CTATTAA2002.5465852E-1128.2871421
CATGGGG13100.027.2662734
GCAGAGT21200.025.9378199
ATGGGTA4400.025.6355135
ATGGGAG4550.024.7903885
AGAGTAC19300.023.86449210-11
Produced by FastQC (version 0.11.2)