FastQCFastQC Report
Sun 14 Apr 2019
SRR6576242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427590
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10790.2523445356533128No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6820.15949858509319675No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5520.129095628990388No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5120.11974087326644682No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5000.11693444654926448No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.11319254425968801No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4600.10757969082532332No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4570.10687808414602774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10350.053.151141
TCAACGC13950.039.4162644
ATCAACG14200.039.0532763
AACGCAG14300.038.4515346
CAACGCA14450.038.052385
GTACATG26900.037.5796971
ACATGGG27300.037.0117533
TACATGG27200.036.828112
TATCAAC16500.033.633092
GTCCTAA702.177726E-433.584511
ATTAGAC702.1837214E-433.5687943
CATGGGT6400.033.0442854
ACGCAGA16700.032.925567
CGCAGAG17500.031.4203958
CGTTATT1252.344459E-730.0987662
GCGTTAT1252.3492794E-730.091721
TACATCG953.74883E-529.6818835
CATGGGG13750.029.394064
CATGGGA10400.028.9208094
ATGGGAG5250.028.645375