FastQCFastQC Report
Sun 14 Apr 2019
SRR6576242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427590
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11250.26310250473584507No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5860.13704717135573796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5780.13517622021094974No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5340.12488598891461447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.12324890666292478No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5050.11810379101475713No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4400.10290231296335274No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4380.10243457517715569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.045.447691
ATGGGTA3750.042.607665
CATGGGT5900.040.6217084
GTACATG25400.040.399361
ACATGGG24750.039.8781473
TACATGG25600.039.843862
TCTATAC550.002715323434.1812673
AACGCAG13900.034.1465346
GGTACCT4150.033.9952358
CAACGCA13700.033.6159635
TCAACGC13800.033.3723684
ATCAACG13850.032.9164353
TAGGACA1151.13581336E-732.69134
GGGTACC4500.031.3254977
CATGGGG12350.031.2023224
TGGGTAC4600.029.626496
ACGCAGA16200.029.005097
GTATACG650.00610482528.9666581
CTAGGAC1354.6238893E-727.8514023
TATCAAC16600.027.7497842
Produced by FastQC (version 0.11.2)