FastQCFastQC Report
Sun 14 Apr 2019
SRR6576245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430214
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.18386198496562176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.12063763615317029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC250.005229465756.4118582
GTATCAA12050.039.4024731
GTACATG24700.034.829181
TACATGG24850.034.8081252
ACATGGG25000.034.5912323
ATCAACG15300.031.3326363
GTAGGAC1501.0659278E-931.3326343
AACGCAG15450.031.0284356
TCAACGC15600.030.7300834
CAACGCA15900.029.8546815
CATGGGA15150.027.6099454
TATCAAC17950.026.4512422
ACGCAGA18350.026.124767
CATGGGC3850.025.6357924
CGCAGAG18900.025.3645158
ATGGGAG6500.025.3071295
CATGGGT6050.024.8589524
GTCCTAA950.001301663224.7420441
ATGGGTA3800.024.7362925
GGGTACC4000.024.6744527
Produced by FastQC (version 0.11.2)