FastQCFastQC Report
Sun 14 Apr 2019
SRR6576247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593658
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11280.19000838866822312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.12380865750987943No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6910.11639698277459412No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6710.1130280397131008No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6450.10864841373315949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.047.532481
TACATGG27500.036.578152
GTACATG28250.036.376791
ACATGGG29150.034.5018773
TCAACGC16400.034.1012734
CAACGCA16600.033.690425
ATCAACG17050.032.8012283
AACGCAG17100.032.7053156
AACGTAC902.5930656E-531.3311446
ATGGGTA5400.029.5905255
CATGGGT7500.028.8246524
GGTATCA6050.028.050041
CATGGGG17750.027.5361024
TGGGTAC5900.027.0828536
TATCAAC21000.026.859792
ACGCAGA21300.026.256387
GGTACCT6100.026.1948918
GGGTACC6300.025.3633087
ATGGGAG5300.024.8284515
CGCAGAG22550.024.8009268
Produced by FastQC (version 0.11.2)