FastQCFastQC Report
Sun 14 Apr 2019
SRR6576248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237287
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7870.3316658729723921No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4860.20481526590162966No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4180.1761579858989325No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3940.16604365178033353No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3440.14497212236658563No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3440.14497212236658563No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3050.12853632942386226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.12853632942386226No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2950.12432202354111266No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2930.12347916236456274No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2860.12052914824663805No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2850.12010771765836308No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2630.110836244716314No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2600.10957195295148914No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2480.10451478589218963No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2460.10367192471563971No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2450.10325049412736476No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2390.100721910597715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA853.8362123E-738.835271
GTATCAA7200.037.9876751
GTACATG14050.036.920161
ATGGGTA4400.036.3167155
ACATGGG14150.036.211123
TACATGG14500.035.661252
CATGGGT4900.035.4883654
GGGTACC4650.034.3642047
TGCACTA550.002713221434.180445
TGGGTAC4600.033.716036
TAACGCC1001.4053603E-632.8986744
AACGCAG8650.032.5998426
GGTACCT4850.031.0090588
CAACGCA9200.030.1400875
TCAACGC9100.029.9548364
ATCAACG9100.029.4445783
TCTTAAG650.006139346428.9280092
ACGCAGA10300.027.8338267
CGCAGAG10650.027.3603978
GTACCTG5500.027.3443539
Produced by FastQC (version 0.11.2)