FastQCFastQC Report
Sun 14 Apr 2019
SRR6576249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214890
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7580.35273861045185906No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4640.21592442645074222No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4060.18893387314439944No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3710.17264647028712365No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3550.16520080040951185No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3240.15077481502163897No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3170.1475173344501838No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2940.13681418400111686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.13681418400111686No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2890.13448741216436316No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2750.12797245102145283No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2740.1275070966541021No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2650.12331890734794547No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2640.12285355298059472No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2630.12238819861324399No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2590.12052678114384102No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2530.1177346549397366No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2410.11215040253152776No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2380.11075433942947553No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2370.11028898506212481No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2350.10935827632742334No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2210.102843315184513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC301.3706331E-462.64821
CAAGTCG250.00523636756.383389
CCGCTAA250.00523636756.383384
GCAAACG503.0064302E-546.9861531
CCGTCGT704.6270015E-640.2738469
CAAACGC608.81543E-539.1551252
TGGGTAC2450.038.356046
ATGGGTA2350.037.9888045
GTAGGAT500.0017014137.588923
ATGGGCA2050.036.672125
GTACATG15100.035.4729921
CATGGGC2550.035.009294
TACATGG15400.034.4768522
GTATTGA550.002716038134.1717451
ACATGGG15300.034.0879973
ACCGTCG702.1828925E-433.561548
CATGGGT3750.032.5770654
TATATAT753.272402E-431.3241022
ATAGAGC600.00415951931.32417
CTAGTCC600.00415951931.32418
Produced by FastQC (version 0.11.2)