FastQCFastQC Report
Sun 14 Apr 2019
SRR6576249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214890
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6860.31923309600260596No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4550.21173623714458562No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.17916143143003396No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3760.17497324212387733No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3380.15728977616454928No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3200.14891339755223604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.1475173344501838No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3050.14193308204197497No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2930.13634882963376613No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2920.1358834752664154No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2820.131229931592908No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2810.13076457722555726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2790.1298338684908558No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2700.12564567918469913No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2490.11587323747033365No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2420.1126157568988785No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2290.10656615012331891No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2270.10563544138861743No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2260.10517008702126669No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2240.10423937828656522No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2210.102843315184513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAG200.002142403770.6502761
ATCTAGG352.9014418E-453.8287851
GTACCGT352.9350328E-453.7035146
TCTAATG450.001013113341.76942
GTACATG15150.036.0635071
TACATGG15350.035.816922
ACATGGG15250.035.743653
CATGGGT3700.034.290424
TGTAATC702.1697792E-433.5959794
GTATCAA9050.033.308421
AGTATAG600.00415760431.3270475
TACTTTT7100.030.44462
GTACTTT7250.029.8842561
CATGGGA6800.029.7146284
AACGCAG9900.029.4284426
TATACTG650.0061492128.9172785
TGGGTAC2650.028.3716666
CAACGCA10600.027.9283585
TCAACGC10600.027.9283584
ACTTTTT7750.027.8911783
Produced by FastQC (version 0.11.2)