FastQCFastQC Report
Sun 14 Apr 2019
SRR6576250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312724
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6280.2008160550517389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.1279083153195789No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3560.11383840063442524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7750.046.095421
TACATGG18300.039.813152
GTACATG18450.039.7442321
ACATGGG18800.038.748093
ATCAACG9750.036.152063
AACGCAG10050.035.5405356
TCAACGC10100.035.3645944
CAACGCA10250.034.8470655
ATAGCCC1405.0749804E-1033.5697753
CATGGGG8400.032.4507834
ACGCAGA11150.031.612797
CGCTACA600.00415414531.3367982
CATGGGA6800.031.1014044
CATGGGT5300.031.0362034
TATCAAC12000.029.7699582
CGCAGAG11950.029.4964528
GTAATAC650.006148895228.921653
GTATAGC2002.5465852E-1128.2031171
GCAGAGT13000.027.8370889
GTCATAG856.80052E-427.6501141
Produced by FastQC (version 0.11.2)