FastQCFastQC Report
Sun 14 Apr 2019
SRR6576250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312724
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5830.1864263695782863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.1627633312441642No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3390.10840229723334315No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.10392550619715787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.056.127821
ATCAACG11400.042.8739133
AACGCAG11350.041.8205836
CAACGCA11500.041.683765
TCAACGC11800.041.0222854
ACGCAGA12850.037.304547
CATGGGT4150.036.238194
TATCAAC13700.036.019142
CGCAGAG13200.035.959378
TACATGG17100.033.804432
GTACATG17200.033.4953351
ACATGGG17750.032.566523
GCAGAGT15050.031.8513839
GTTCTAC804.6425452E-429.5143281
GAGTATA650.006006885329.0602631
GCGATGT700.00882966126.8550878
TAATGCG350.008836294526.85508718-19
AGAGTAC13950.026.6144510-11
GTATTGG1109.838445E-525.7579611
CATGGGA7150.024.9771084
Produced by FastQC (version 0.11.2)