FastQCFastQC Report
Sun 14 Apr 2019
SRR6576251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283632
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.20801602075929373No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5790.2041377559654764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3420.12057877813504825No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3360.11846336097478423No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3230.11387995712754556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.10295030179951488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.043.212191
TACATGG15500.034.2576372
CCCTTAC550.00268817234.2474251
GTATAAG1107.5333446E-834.2474251
GTACATG15550.034.21991
ACATGGG15550.034.1474883
AACGCAG11300.032.8518146
CATGGGG6800.032.478774
CAACGCA11450.032.011045
TCAACGC11450.032.011044
ATCAACG11550.031.733893
CATGGGT4000.031.718634
GAGTGCG600.004159830531.3270447
CTAGTAC1252.356719E-730.0739653
GTATTAT804.7186113E-429.4313811
AGAACCG650.006152490628.9172695
CTCGATA505.326126E-528.19433864-65
TATCAAC13300.027.9116922
ATGGGAG2700.027.8462625
ACGCAGA13200.027.7671537
Produced by FastQC (version 0.11.2)