FastQCFastQC Report
Sun 14 Apr 2019
SRR6576252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541391
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9680.17879868708567376No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6230.1150739484032797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.1095326667787237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12650.044.2123951
TACATGG29850.038.2604032
GTACATG30400.038.0319981
ACATGGG30750.036.9877473
CATGGGT7800.035.5504464
ATGGGTA4200.034.6896555
ATCAACG16300.033.158733
AACGCAG16850.032.9131746
TCAACGC16600.032.5594754
CAACGCA16650.032.4616975
GGGTACC3650.029.615747
CATGGGA16550.029.5340484
ATGGGAG8000.029.3743065
TATCAAC19100.029.0359632
ACGCAGA19900.027.868697
GGTACCT4300.027.3249388
CGCAGAG20500.027.053028
TGGGTAC4100.026.3652346
CATGGGG10300.026.0090944
GCGGTAT1659.3008566E-825.6357571
Produced by FastQC (version 0.11.2)