FastQCFastQC Report
Sun 14 Apr 2019
SRR6576254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407457
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7640.18750444832215424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5890.14455513097087544No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4860.11927638990126566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.044.289361
TACATGG24450.039.603582
GTACATG25200.039.1710171
ACATGGG25850.037.272293
AACGCAG14700.033.2513126
TCAACGC14700.033.2513124
ATCAACG14750.032.8199543
CAACGCA14900.032.8049855
CATGGGA13750.031.1050874
CATGGGG9450.030.3382664
TATCAAC16950.029.6729092
AACGTTC650.00614992328.9227377
ACGCAGA17250.028.33597
CATGGGT5000.027.259684
CGCAGAG17950.026.9690428
ATGGGAG7050.026.6663535
ATGGGTA4500.026.1108065
GGTATCA5800.025.933911
CATGGGC4800.024.4788824
GCAGAGT20850.023.6687899
Produced by FastQC (version 0.11.2)