FastQCFastQC Report
Sun 14 Apr 2019
SRR6576255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384092
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7910.20594024348333215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.14059131666371597No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4810.1252304135467544No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4760.12392864209616447No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4390.11429553336179873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCG352.9391627E-453.6990367
GTATCAA11150.051.41811
ATCAACG14950.038.0293353
TCAACGC15100.037.6515584
AACGCAG15450.037.102736
CAACGCA15550.036.864135
GTACATG25000.034.962621
TACATGG25300.033.990822
ACATGGG25600.033.4045753
TATCAAC17750.031.7697622
ACGCAGA18400.030.8988327
CGCAGAG19050.029.8445428
CATGGGG8950.027.8245014
CATGGGC4000.027.0173264
CGAGTCG350.00884647226.84951842-43
GTAATAC1057.423265E-526.8495183
CATGGGA14500.026.5717644
CATGGGT5150.026.4585064
GCAGAGT21750.026.3557329
ATGGGTA3300.025.6290865
Produced by FastQC (version 0.11.2)