FastQCFastQC Report
Sun 14 Apr 2019
SRR6576255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384092
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7860.20463847203274216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.14788123678701978No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.10960915613967487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.043.0929261
CGTATAG500.001692391337.6371231
TACATGG24550.035.0281222
GTACATG25100.034.6756321
ATCAACG14350.034.0563963
ACATGGG25300.033.6182673
TCAACGC14800.033.3384064
CAACGCA14950.033.318235
AACGCAG15200.033.0836946
CCGACCT600.004145454631.3520099
CATGGGA13750.031.0996824
CATGGGT4700.030.9942474
GGGTACC2750.030.7619327
ATGGGTA2950.030.2655685
ATGGGAG7350.030.0488435
TATCAAC17250.029.1482122
ACGTTAA650.00613125828.9403158
CGCAGAG17500.028.754278
ACGCAGA17650.028.491347
TGGGTAC3450.027.2448676
Produced by FastQC (version 0.11.2)