FastQCFastQC Report
Sun 14 Apr 2019
SRR6576256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355436
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.1960971876793572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.13785885504000722No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4170.11732069908506737No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3730.10494153659167894No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3610.1015654013662094No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3590.10100271216196446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC405.67215E-446.9987343
TAGAGCG555.276544E-542.726125
GTACATG26400.042.5600741
TACATGG26450.041.94642
ACATGGG26400.041.8363
GTATCAA10050.040.6969221
CATGGGA14000.037.2632834
ATGGGAG6250.036.8470045
ATCAACG12800.031.2100963
CATGGGT6350.031.0857774
CATGGGG9250.030.993764
TCAACGC12950.030.848594
AACGCAG13000.030.729946
CAACGCA13350.029.9242885
GTATAGG953.7385653E-529.6917671
TATCAAC14500.028.2071782
CATGGGC4250.027.6463154
TACTTAG856.807834E-427.6463137
TCTAGTC856.807834E-427.6463133
TAGTACT909.5061946E-426.1104094
Produced by FastQC (version 0.11.2)