FastQCFastQC Report
Sun 14 Apr 2019
SRR6576256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355436
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6990.19665987688360212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.14545515929731373No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4230.11900876669780212No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3870.10888036102139345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG156.899284E-493.9964759
GTATCAA12200.043.2676731
TACATGG26750.040.9425432
GTACATG26950.040.2229921
TACTACC608.8064204E-539.1707083
ACATGGG27250.038.983843
AACGCAG15250.034.5215956
CATGGGT6150.034.3937954
ATCAACG15850.033.2147873
TCAACGC16050.032.8008964
CAACGCA16050.032.508035
CATGGGA14550.031.9826854
AACAACG600.004157912531.3321597
CATGGGG9250.030.9977974
ACGCAGA17300.030.154947
ATGGGTA3600.030.0308785
TATCAAC17750.029.9242172
GTATACG650.006065555429.0036031
CGCAGAG18200.028.405538
ATGGGAG6300.028.3521355
Produced by FastQC (version 0.11.2)