FastQCFastQC Report
Sun 14 Apr 2019
SRR6576258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547157
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.12299943160738143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16100.035.636941
CAACGCA19100.029.7742335
AACGCAG19450.029.4800876
TCAACGC19300.029.4656934
TACCGTA804.7782445E-429.3743747
ATCAACG19450.029.2384473
CATGGGG15300.028.87524
GTACCGT1154.1918065E-628.6080846
ACATGGG28700.028.4941623
TACATGG28900.028.1369172
TATCAAC20450.028.0410842
GTACATG28950.027.9412821
CCGTATG856.8123447E-427.6464679
TACGGGC1057.4156684E-526.856574
ATGGGGA8000.026.4369345
ACGCAGA22550.025.4273977
CGCAGAG23000.024.725568
GTACTAG1452.5180712E-522.7036911
ACTCGTC1252.4215336E-422.5595178
GCAGAGT26150.021.9268729
Produced by FastQC (version 0.11.2)