FastQCFastQC Report
Sun 14 Apr 2019
SRR6576260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270698
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6630.24492238583218198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.1507214682044197No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4080.1507214682044197No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3420.12634005423017533No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3380.12486239277719083No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3090.11414934724305315No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3040.11230227042682252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGT652.7514416E-643.4429361
TATACTG1253.6379788E-1241.3543935
GTATCAA9200.037.855171
AACGCAG11300.030.3587136
GTACATG15600.029.867021
GTATAGG953.707636E-529.7241151
CAACGCA11600.029.5735765
ATCAACG11800.029.0723293
TCAACGC11800.029.0723294
GCTATAT650.00615004628.9191553
CTACGTG650.00615004628.9191554
TACATGG16300.028.5421412
ACATGGG16250.027.762393
CCTAGTA1057.407823E-526.85352
ATAGACT700.00883014426.85354
GGACCAT1258.002744E-626.3164316
GAGCTAT909.424601E-426.1462121
AGCCGAG909.506348E-426.1075697
ACGCAGA13150.026.0877157
CGCAGAG13250.025.9051868
Produced by FastQC (version 0.11.2)