FastQCFastQC Report
Sun 14 Apr 2019
SRR6576261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706040
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17700.2506940116707269No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10420.14758370630559176No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9360.1325703926123166No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9250.1310124072290522No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8850.12534700583536346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.10197722508639737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16750.045.023041
GTACATG39200.036.191791
TCAACGC20700.035.8794484
ATCAACG21050.035.0595743
TACATGG40000.035.019932
CAACGCA21350.035.0047955
AACGCAG21350.035.0047956
ACATGGG40550.034.429023
CATGGGT9700.032.468494
TATTCCG600.004158477331.3355275
ACCGTAT753.2756463E-431.331098
TACCGTA953.7520804E-529.6820857
CATGGGG20650.029.5925464
ACGCAGA24950.029.5730347
TATCAAC26100.028.4561162
CGCAGAG26000.028.197988
ATGGGTA7100.027.8047665
GTCTATA909.357795E-426.1852741
GTACCGT1458.637253E-725.9328526
GGTACCT6600.025.6345278
Produced by FastQC (version 0.11.2)