FastQCFastQC Report
Sun 14 Apr 2019
SRR6576262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648761
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18150.2797640425364657No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9720.1498240492261403No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9040.13934253137904404No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8720.13441005239217524No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8630.1330227926771184No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7070.10897695761613291No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.10866867767945361No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6780.10450689853428305No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6720.10358205872424513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15050.048.1390531
CCGTATG503.000568E-547.019589
TACCGTA503.0101532E-546.9942177
ACACGAC608.826227E-539.161853
ACATGGG36250.035.7804263
GTACATG37000.035.601611
TACATGG37150.035.5460972
GTACCGT1204.2055035E-935.2456636
TCAACGC20850.034.484974
ATCAACG21300.033.756413
CAACGCA21450.033.520355
AACGCAG21700.033.350746
ACCGTAT851.7486149E-533.19038
CATGGGT9850.032.919814
ATGGGTA8200.031.5205145
ACGCAGA24500.029.7310377
CGCAGAG24850.028.9496848
GGGTACC8550.028.581287
CATGGGG17700.028.1434294
GGTACCT8950.027.8448