FastQCFastQC Report
Sun 14 Apr 2019
SRR6576263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154894
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3610.23306261055947938No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1940.12524694307074513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.12201892907407645No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1680.10846127028806797No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1640.10587885909073302No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1610.1039420506927318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA255.4136886E-575.56211
GAAGTAT405.528858E-447.2263151
GTATCAA5000.044.3927351
GTATGGG500.001657875537.781051
AACGCAG5750.037.5980646
GGGTAAA905.917209E-736.731581
ATCAACG5950.036.3342633
CAACGCA6100.035.4407965
TACATGG11300.034.9362532
GTACATG11250.034.8425251
TCAACGC6100.034.6703454
TATAGAC550.002710472334.1800582
ACATGGG11650.033.4832533
TATCAAC6800.031.7924792
CGCAGAG6900.031.3317188
TATCATG300.00415738131.33171752-53
CATATAC600.00415102131.3317173
TAATTGA600.00415102131.3317174
AACTACT600.00415102131.3317177
CATGGGG3800.030.919464
Produced by FastQC (version 0.11.2)