FastQCFastQC Report
Sun 14 Apr 2019
SRR6576264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139051
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3200.230131390640844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.1366405131930011No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1850.13304471021423794No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1770.12729142544821684No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1720.12369562246945366No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1690.12153814068219573No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1480.10643576817139036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG405.646504E-447.021771
ATGGGAT1500.040.7228935
ACATGGG10500.040.2753873
TACATGG10550.040.1133582
GTACATG10850.039.4376141
CATGGGT3350.039.2735144
GTATCAA5050.039.1072161
GTACAGA1152.7248461E-936.7996441
ATGGGAG2450.036.439645
CCTACAC651.4092341E-436.144583
CATGGGC1451.8189894E-1135.6460344
ATCAACG5500.035.027383
TCAACGC5750.033.504454
CATGGGA5050.033.4963654
CCGATCG851.7468257E-533.167979
CAACGCA5700.032.9745
AACGCAG5750.032.687276
GGGTACC1457.3305273E-1032.4054877
GTATAGC753.2520157E-431.3478471
GCCGATC902.582208E-531.3253068
Produced by FastQC (version 0.11.2)