FastQCFastQC Report
Sun 14 Apr 2019
SRR6576264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139051
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3510.2524253691091758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.17835182774665412No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1850.13304471021423794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720.12369562246945366No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1590.11434653472466937No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1540.11075073174590619No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1540.11075073174590619No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1520.10931241055440091No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1470.10571660757563771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG10200.041.9209483
GTACATG10450.041.4272881
TACATGG10700.040.4011652
ATGGGAT952.098568E-839.5691075
CTAACAC500.001698961837.5906523
GTATCAA4300.037.2070661
CATGGGA5100.035.932244
CATGGGT2450.032.6041374
CATGGGG3550.031.7667484
GGGTTAG600.00412440531.3706651
CTACGAG600.004131683631.3593794
ATGGGTA1501.0550139E-931.325545
TATTAAG753.2656288E-431.325542
CATGGGC1751.6916601E-1029.5355114
GTATTAA804.726448E-429.4099981
GTATAGG650.006100217328.9575351
GGTCTGG650.006100217328.9575351
ATGGGCA1303.3033393E-728.9158845
GTTAGAG650.006143294728.9158843
CTACACT650.006143294728.9158844