FastQCFastQC Report
Sun 14 Apr 2019
SRR6576265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169006
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3500.20709323929327955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.16508289646521423No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2330.13786492787238322No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2310.1366815379335645No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1940.1147888240654178No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1930.11419712909600842No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1740.1029549246772304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGGT250.00522885956.3985827
CATGGGT2900.040.516224
CATGGGG3750.040.105664
ACATGGG13800.036.7816853
GTACATG14150.036.6228641
ATATAGT651.4086037E-436.152944
TACATGG14200.036.0765532
ATGGGTA1750.034.9134065
GTATCAA7100.034.503331
GGTACCT1256.200935E-933.8391468
CGACCCG902.5814008E-531.3325445
AATATAG600.0041515531.3325443
CTATCTG804.7645476E-429.374269
CCATAAT804.7645476E-429.374263
CATAATG650.00614028328.9223524
ACTCCCT650.00614028328.9223528
AACGCAG8450.028.3661526
GTACTTT5650.028.3495731
GGGTACC1503.6596248E-828.199297
ATGGGTG1354.5964043E-727.851155
Produced by FastQC (version 0.11.2)