FastQCFastQC Report
Sun 14 Apr 2019
SRR6576267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214877
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4280.19918371905787965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.10191877213475617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2190.10191877213475617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG250.005230264356.3999987
GTATCAA7650.041.16341
GTACATG15550.040.501611
CATGGGT3200.039.6562464
ACATGGG15650.039.3418543
TACATGG15800.039.2658232
AACGCAG8500.037.047066
ATGGGAT2300.036.7826085
ATCAACG8650.036.4046253
CAACGCA8900.035.3820235
TCAACGC8900.035.3820234
GGTACCT1651.8189894E-1234.181828
ATAGTAC550.002712117334.1818163
ATGGGTA2250.033.4222225
ACGCAGA9600.032.8020827
GGGTACC1755.456968E-1232.2285737
CGCAGAG9950.031.6482418
CATGGGG4950.031.3333364
TATCAAC10050.031.3333342
CATGGGA9000.031.3333344
Produced by FastQC (version 0.11.2)