FastQCFastQC Report
Sun 14 Apr 2019
SRR6576267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214877
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3990.18568762594414479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.12286098558710332No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2180.10145338961359289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC250.005230623356.3990179
ATGGGAG3600.041.7770545
GTATCAA6500.040.557681
TACATGG14300.037.4678842
GTACATG14450.037.465231
ACATGGG14350.037.009813
AACGCAG7900.033.9108056
TGGGTAC2100.033.5708476
CAACGCA7900.033.315885
ATCAACG7900.033.315883
GGTACCT1850.033.0264558
TCAACGC8150.032.293924
CATGGGT2950.031.8638534
CATGGGA9000.031.8550014
CATTCGC300.004158462431.33278880-81
CATGGGG4550.030.9884724
GGGTACC2000.030.5494697
CCCTAAA804.7232586E-429.4224221
ACGCAGA8950.029.4073127
ATAACCC650.006143708728.9225753
Produced by FastQC (version 0.11.2)