FastQCFastQC Report
Sun 14 Apr 2019
SRR6576268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201937
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4090.2025384154464016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2810.1391523098788236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11488731634123515No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2140.10597364524579447No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2050.10151680969807414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTC451.6120885E-552.2062645
ATATACT906.1714127E-736.5443844
GGGTACC1850.035.5566987
ATGGGAG3100.034.860315
ATGGGTA2050.034.3797345
GTATCAA8800.034.1883051
ATACTCT702.182568E-433.5611726
TTAACCT702.182568E-433.5611724
GTACTTG851.7453875E-533.182771
GGTACCT2000.032.8899468
GTATACT600.00415915331.3237574
CGGTAAG300.00416403831.32375780-81
ACATACC600.00415915331.3237574
GTACATG16100.031.2419531
TACATGG16000.031.143412
ACATGGG15700.030.5257023
CATGGGG4300.029.502614
GGTCGGA804.7756347E-429.3660247
GTCGGAA804.7756347E-429.3660248
CATGGGT3550.029.117864
Produced by FastQC (version 0.11.2)