FastQCFastQC Report
Sun 14 Apr 2019
SRR6576269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656371
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10580.16118932737735214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.10710406157493246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.049.410351
GTACATG30700.035.853571
TACATGG30700.035.3696372
TCAACGC15950.034.7705964
CAACGCA16200.034.2340135
ACATGGG31300.034.085713
ATCAACG16450.033.999453
AACGCAG16800.033.2911266
TATCAAC19400.029.5607262
CGTCTAG650.00612760728.9468651
CATGGGA15250.028.353644
ACGCAGA20100.027.591597
CGCAGAG20500.027.0532238
ATGGGTA5550.025.4049955
CATGGGG16400.024.6459434
CATGGGT8200.024.0727834
ATGGGAG8100.023.7897385
GGTATCA5750.023.723841
GCAGAGT23600.023.4996199
GGTACCT5500.023.0723518
Produced by FastQC (version 0.11.2)