FastQCFastQC Report
Sun 14 Apr 2019
SRR6576271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228350
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4210.18436610466389317No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2410.10553974162469894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG250.00523102156.3990825
GCACCGT405.667567E-446.9992376
ATGGGTA1500.040.732675
GTATCAA4850.038.7622531
TAGGGGC500.00169930937.599397
CATGGGT2500.037.5993844
TAACGCC651.4100813E-436.153264
GTACATG12200.035.8272861
TACATGG12500.034.2154432
ACATGGG12350.033.8698923
ATACTAT702.179754E-433.570881
CAACGCA6000.031.3328235
ATACTGC1052.056062E-631.3328236
CTGTTAG753.2677047E-431.3328231
GTGTAAG902.585191E-531.3328231
TCAACGC6000.031.3328234
CCTTAAT600.00415439831.332821
ACGCCGC600.00415439831.332826
ATCAACG6150.030.5686073
TGGGTAC2000.030.5495036
Produced by FastQC (version 0.11.2)