FastQCFastQC Report
Sun 14 Apr 2019
SRR6576271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228350
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4260.18655572585942634No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2290.10028465075541931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC503.0023759E-546.998478
GGTACCT2050.043.559558
CATGGGT3350.039.2909054
CCTACAC608.792218E-539.1739653
GGGTACC2400.039.1653867
ATGGGTA2350.038.0070845
CCGACCA757.419121E-637.5987749
TTCCGAC651.4102168E-436.1526687
TACATGG14650.035.296682
GTACATG14800.035.0002671
ACATGGG14900.034.7044533
TGGGTAC2600.034.3525546
ATAGAGT550.002709993634.188196
GTATCAA6000.033.7487451
GGTGTAA753.2304123E-431.394181
CTAGCGG300.00415904531.3323118-19
AATACGA650.006138392728.9284672
CACTCTA1951.8189894E-1128.9221349
CATGGGG4950.028.4901584
CAATACG700.008738372526.9092961
Produced by FastQC (version 0.11.2)