FastQCFastQC Report
Sun 14 Apr 2019
SRR6576272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212153
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3930.1852436684845371No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2600.12255306311954109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT450.001013797441.7634284
TACATGG13100.037.7032472
GTACATG13250.037.2852131
ACATGGG14000.035.909093
GTTTAAG550.002697820834.2184641
CATGGGG4400.034.170084
ATGGGAG2750.034.170085
TAGGTTG550.002716612334.170085
GTGCTAC600.004131670631.3669261
TACACCT902.5901174E-531.322574
ATAGTAC600.004160397731.3225693
GTATCAA6600.029.9411561
CATGGGA7750.029.0996784
ATGGGTA1951.8189894E-1128.9131435
TGGGTAC1951.8189894E-1128.9131436
CATGGGT3600.028.7123574
CTCTTAC700.00877408926.8859371
GGCTTAC700.00877408926.8859371
ACTATTC700.00883487326.8479168
GGTACCT2104.9112714E-1126.8479168
Produced by FastQC (version 0.11.2)