FastQCFastQC Report
Sun 14 Apr 2019
SRR6576274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323625
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9600.2966396292004635No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5630.1739667825415218No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5110.15789880262649672No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4800.14831981460023175No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4390.13565083043646198No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4260.13163383545770568No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4080.12607184241019698No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3960.1223638470451912No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3910.12081884897643877No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3870.11958285052143686No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3770.11649285438393202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.11123986095017381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3550.1096948628814214No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3540.1093858632676709No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3400.10505986867516415No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3380.10444186944766319No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3370.10413286983391269No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3360.10382387022016223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9250.046.3176351
GTACATG19100.038.207191
ATCAACG11200.037.3433043
TACATGG19600.037.163482
TCAACGC11350.036.849784
ACATGGG19550.036.2970163
AACGCAG11400.036.2759326
CAACGCA11550.035.3979455
GTATTAC550.002691281334.240811
GTATATG702.1595591E-433.6293641
CATGGGT5500.033.322894
ATGGGTA4100.033.2395325
CATGGGA7250.031.113154
ACGCAGA13350.030.2731727
CATGGGG8850.030.2672064
ACCGTGC1102.9485545E-629.9236538
CGCAGAG13650.029.6261398
GGTACCT4350.029.1866178
TATCAAC14350.029.1459922
TGGGTAC4400.028.8371166
Produced by FastQC (version 0.11.2)