FastQCFastQC Report
Sun 14 Apr 2019
SRR6576275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229785
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3770.16406640990491111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.1209826577017647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2390.10401027047022217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG16500.037.7342951
TACATGG16600.037.3844872
ACATGGG16500.036.7562643
ATGGGAC1850.033.0367435
CATGGGT3650.032.2012524
CATGGGG5200.031.643924
GTAAGAG600.0041481431.3425483
CATGGGA9600.030.8528214
TAGACTG1102.9480689E-629.9178895
GTCTTAG953.6494253E-529.7902321
ATGGGTA2151.8189894E-1228.4269645
GTACTTT6950.027.825651
ATGGGAG3950.027.375145
TCTATAC700.00880892826.8650463
GTATCAA7750.026.7791791
TACTTTT7600.026.5999262
GTACCTG1956.002665E-1026.5090779
ATGGGGG1807.408744E-926.1187935
GGTACCT2008.058123E-1025.846358
GGGTACC2051.0713848E-925.2159527
Produced by FastQC (version 0.11.2)