FastQCFastQC Report
Sun 14 Apr 2019
SRR6576276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209474
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3270.15610529230357945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.10932144323400517No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2250.10741189837402255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG15750.037.616341
CCGCTAA500.00170120637.58944
CGCTAAA500.00170120637.58945
TACATGG16250.036.748272
ACATGGG16300.034.8797343
CCCGCTA550.002715713334.172183
CATGGGT3200.033.771734
GTAATAC702.1825416E-433.5619663
ATGGGAG3550.033.089265
TGCCCGC753.2581546E-431.346951
TAGCGTA600.004159022631.32457
GGTACCT1401.8806531E-830.205778
GTATCAA7700.029.9220891
CATGGGA9150.029.2704354
TAATACT1303.317855E-728.9149254
TAGACAC650.00615130728.9149255
GCGTATA650.00615130728.9149259
GTATAAT1005.28737E-528.2122551
GTCTTAG856.7801046E-427.6590731
GACTGTC856.8085757E-427.6392657
Produced by FastQC (version 0.11.2)