FastQCFastQC Report
Sun 14 Apr 2019
SRR6576276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209474
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3660.17472335468841002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.14751234043365763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2160.10311542243906166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG15800.039.0826151
TACATGG15650.039.0344962
GTATCAA8200.038.515041
GATATAC500.001700357237.593223
ACATGGG16700.036.2988433
CATGGGT3150.035.8030664
TGGGTAC1800.033.9383246
AACGCAG8750.033.2968526
GTATATA1308.85484E-932.633871
CAACGCA9200.032.1789785
ATCAACG9350.031.6627393
GGGTACC1950.031.3276847
TCAACGC9450.031.3276844
ATGGGAG3200.030.838195
CATGGGG5750.030.2380264
TATCAAC10150.029.6301252
CATGGGA9550.029.523474
CTAAATG650.006148255428.9178623
TATAACC650.006148255428.9178623
CTAGGAC650.006148255428.9178623
Produced by FastQC (version 0.11.2)