FastQCFastQC Report
Sun 14 Apr 2019
SRR6576277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6550.26740806061793715No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3200.13064210595074793No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3130.12778430988307532No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2860.11676138219348095No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2770.11308707296361618No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2590.1057384545038866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2590.1057384545038866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC405.668045E-446.999793
TAGTACT405.668045E-446.999794
ACATGGG18650.040.5735473
TACATGG19000.039.8342742
GTACATG19100.039.6257171
CATGGGG5700.037.9296574
ATGGGAG3750.033.839855
GCATAGG702.1774467E-433.5781361
CATGGGT4200.033.571284
GTATCAA8500.033.18311
GGTACCT2350.031.9998598
CATGGGA10800.031.7683814
TAGGGCC600.00415473831.3331994
ATGGGTA2550.031.3331975
ATGGGAT2700.031.3331975
ATGGGGG2300.030.652045
TAGCGCG1102.9559924E-629.908964
GTAGCGC1102.9559924E-629.908963
CGCGCGT1205.8188034E-627.4165487
AACGCAG9950.027.3968646
Produced by FastQC (version 0.11.2)