FastQCFastQC Report
Sun 14 Apr 2019
SRR6576278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222787
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5440.2441794180091298No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3090.13869750030297998No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2770.12433400512597234No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2710.12164084978028342No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2650.11894769443459448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2580.11580567986462405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.11266366529465363No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2450.1099705099489647No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2380.10682849537899429No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2270.1018910439118979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC250.005236656.3834349
GTACATG18300.040.0717961
TACATGG18600.038.9287642
ACATGGG19100.038.6308333
ATGGGAG3700.036.8352855
CATGGGC3350.033.669314
CATGGGA9850.033.39874
GTATCAA8500.033.181621
CATGGGG6750.032.027414
ATGGGAT2350.031.9977845
ATGGGTA2000.030.5478845
ATGGGGA2950.030.2690895
CTATAGA804.7643215E-429.379561
CATTAGA650.006132269728.9340632
ATGGGGG2600.028.9210745
TAGACTG650.006145745528.9210745
GTTGCAC650.006145745528.9210743
CCTATTC1354.6092646E-727.8499223
CGACGGT856.8014767E-427.6451447
CTAACCT700.0088239926.8552824